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SECOND INTERNATIONAL SYMPOSIUM
ON THE ROLE OF SOY
IN PREVENTING AND TREATING CHRONIC DISEASESeptember 15-18, 1996
Brussells, Belgium
POSTER ABSTRACTSDietary Genistein Inhibits Protein Tyrosine Phosphorylation in the Dorsolateral Prostate of the Rat.
Abraham Dalu; Joyce Haskell and Coral A. Lamartiniere. Department of Pharmacology and Toxicology. University of Alabama at Birmingham, Birmingham, Alabama 35294-0019 USA.
Prostate cancer is one of the major malignancies of men in the Western World. In the U.S. from 1958-1967, the increased mortality rate was 9.1/100,000 in white men and 29.0/100,000 in non-white men. In 1996, over 140,000 men will be diagnosed with prostatic cancer and 33,000 will die from this cancer. Yet, Asian men, consuming a traditional diet high in soy products have a low incidence of prostate cancer. Asians who emigrate to the United States and adopt a western diet lose this protection. One of the components of soy, genistein has been reported to inhibit protein tyrosine phosphorylation in vitro. Protein phosphorylation is associated with cell proliferation and its inhibition with potential for cancer prevention/therapy. Accordingly we have investigated the potential of genistein in the diet, to inhibit tyrosine phosphorylation in the prostate of Lobund-Wistar rats. The Lobund- Wistar rat is the most susceptible animal model for spontaneously developing and chemically-induced prostate cancer. Five to six week old male Lobund-Wistar rats were fed AIN_76A diet containing zero, 0.025-, 0.25- and 1.0 mg genistein/g diet. The 1 mg genistein/g diet resulted in decreases in weights of the dorsolateral- and ventral prostates, but not of the testes, seminal vesicle and epididymis. The dorsolateral prostates were homogenized and immunoprecipitated with anti-phoshpotyrosine antibody. Western blot analysis revealed that increasing dosed of genistein-in-the-diet inhibited the expression of tyrosine phosphorylated proteins with molecular weights of approximately 180,000, 85,000 and 45,000. These results are supportive of the hypothesis that genistein could protect against prostate cancer by inhibiting protein kinase activity in vivo.
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