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SECOND INTERNATIONAL SYMPOSIUM
ON THE ROLE OF SOY
IN PREVENTING AND TREATING CHRONIC DISEASESeptember 15-18, 1996
Brussells, Belgium
SCIENTIFIC PROGRAM
(Oral Abstracts)Soy and Cancer
Animal studiesEffects of Dietary Soy Protein Isolates (SPI), Genistein and 1,4-Phenylenebis (Methylene) Selenocyanate (p-XSC) on the Levels of 7, 12-Dimethyl-benz[a] Anthracene (DMBA)-DNA Binding in Mammary Glands of Female CD Rats
Pramod Upadhyaya and Karam El-Bayoumy, American Health Foundation, Valhalla, NY 10595.Epidemiological studies and assays performed in laboratory animals point to the value of soybean and selenium in the prevention of breast cancer. Our preliminary results indicate that a diet supplemented with SPI (10%), genistein (111 ppm), or selenium in the form of p-XSC (5 ppm as Se) inhibited total DMBA-DNA binding and individual (DMBA-DNA adduct formation in the mammary tissues of rats. Genistein and p-XSC appear to be better inhibitors than SPI.
Literature data suggest that the simultaneous administration of more than one chemopreventive agent is a promising approach in cancer prevention. Therefore, we examined the effect of genistein at two dose levels and of p-XSC administered individually or in combination on DMBA-DNA adduct formation in the mammary tissues of female CD rats. A semipurified high-fat diet containing genistein (111 or 222 ppm), p-XSC (5 ppm as Se) or a combination of p-XSC (5 ppm as Se) and genistein (111 ppm) was fed to 6-weeks-old virgin female CD rats for 1 week before carcinogen treatment. At 7 weeks of age, 9 rats/group were given a single dose of [3H]DMBA by gavage (5 mg/rat, 0.2 ml olive oil, specific activity 51.2 mCi/mmol) and were sacrificed 24 hours later. There was no effect on the levels of DMBA-DNA binding in the liver. However, the combination of p-XSC and genistein significantly inhibited total DMBA-DNA binding as well as individual DMBA-DNA adduct formation in the mammary tissues and appears to be more effective as an inhibitor than either agent alone.
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